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Technical Manufacturing Company dota–bn–tmc–mnps
Dota–Bn–Tmc–Mnps, supplied by Technical Manufacturing Company, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dota%E2%80%93bn%E2%80%93tmc%E2%80%93mnps/dota+bn+tmc+mnps/pm34906606-383-12-7
Average 90 stars, based on 1 article reviews

dota–bn–tmc–mnps - by Bioz Stars, 2026-09

90/100 stars

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In Vitro:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Peptide conjugation efficiency to NPs has been estimated using UV–VIS spectroscopy and Bradford protein assay.40,41 Figure 10 Phantom study, MrI, and PeT/cT of DOTa–BN–TMc–MNPs and 68ga–DOTa–BN–TMc–MNPs. (A) MrI phantom study of serially diluted aqueous solutions of 200 μM DOTa–BN–TMc–MNPs for Fe and (B) relaxation rate (1/T2) as a function of iron concentration.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Article Title: Chitosan: A versatile bio-platform for breast cancer theranostics.
Article Snippet: Breast cancer is considered one of the utmost neoplastic diseases globally, with a high death rate of patients.. Over the last decades, many approaches have been studied to early diagnose and treat it, such as chemotherapy, hormone therapy, immunotherapy, and MRI and biomarker tests; do not show the optimal efficacy.. These existing approaches are accompanied by severe side effects, thus recognizing these challenges, a great effort has been done to find out the new remedies for breast cancer.

Cell Binding Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Binding Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Concentration Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Saturation Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Sequencing:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Suspension:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

X-ray Diffraction:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Cell Culture:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Cytotoxicity Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

MTT Assay:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Radioactivity:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Software:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Positron Emission Tomography:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

Centrifugation:

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: 94 .1 73 .2 22 o n 06 -M ay -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 International Journal of Nanomedicine 2019:14submit your manuscript | www.dovepress.com Dovepress Dovepress 2594 hajiramezanali et al (DOTA–BN–TMC–MNPs) were precipitated by centrifugation (30,000 rpm, 30 minutes) and washed with water.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In order to evaluate the IC 50 value of DOTA–BN–TMC– MNPs, T-47D cells were aliquoted into 2 mL microcentrifuged tubes (1×105 cells), and the 99mTc–HYNIC–GABA–BN (7–14) (100 μL, 240 nM) cells were added, followed by the addition of various concentrations of DOTA–BN–TMC–MNPs (0.001–60 μg/mL, 46.8 pM–2.81 μM BN) in triplicate.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: In vitro competitive cell-binding assay Competitive cell-binding assay was used to evaluate the IC 50 value of DOTA–BN–TMC–MNPs in GRP receptorexpressing human BC cell line T-47D.33 The IC 50 value was determined against 99mTc–HYNIC–gamma-aminobutyric acid (GABA)–BN (7–14) as a standard GRP receptor binding agent.34 In order to measure the IC 50 value, the total concentration of receptors expressed on T-47D cells (B max ) was determined using the saturation binding assay.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The IC 50 value of DOTA–BN–TMC–MNPs was 8.79 μg/mL (411.1 nM for BN).

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: The amino acid sequence of BN in this kit contains the C-terminal region of BN, which is the active site and has a high binding affinity to GRP receptors.48,49 The obtained IC 50 value for DOTA–BN–TMC–MNPs is 8.79 μg/mL (411.1 nM for BN), and it is assumed that DOTA–BN–TMC–MNPs have great ability to compete with radiopeptides toward GRP receptors.

Article Title: 68Ga-radiolabeled bombesin-conjugated to trimethyl chitosan-coated superparamagnetic nanoparticles for molecular imaging: preparation, characterization and biological evaluation
Article Snippet: Small hydrodynamic size, low toxicity, highly efficient radiolabeling with 68Ga, high serum stability, and strong binding affinity (IC 50 =8.79 μg/mL) of DOTA–BN–TMC–MNPs toward GRP receptors make these NPs suitable for dual-modality PET/MRI of prostate, breast, and lung cancers.

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dota–bn–tmc–mnps (Technical Manufacturing Company)

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Cell Binding Assay:

Binding Assay:

Concentration Assay:

Saturation Assay:

Sequencing:

Suspension:

X-ray Diffraction:

Cell Culture:

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MTT Assay:

Radioactivity:

Software:

Positron Emission Tomography:

Centrifugation:

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